![Reply to Behnam and Klein: Potential role of the His-tag in C-terminal His- tagged SARS-CoV-2 main protease | PNAS Reply to Behnam and Klein: Potential role of the His-tag in C-terminal His- tagged SARS-CoV-2 main protease | PNAS](https://www.pnas.org/cms/10.1073/pnas.2108209118/asset/db162737-c211-407e-a14b-dff47986d92a/assets/images/large/pnas.2108209118fig01.jpg)
Reply to Behnam and Klein: Potential role of the His-tag in C-terminal His- tagged SARS-CoV-2 main protease | PNAS
![E.coli Plasmid #10 (N-terminal signal peptide for membrane protein expression, C-terminal CL7, N-terminal HIS ) • NUCLEUS BIOTECH E.coli Plasmid #10 (N-terminal signal peptide for membrane protein expression, C-terminal CL7, N-terminal HIS ) • NUCLEUS BIOTECH](https://nucleusbiotech.com/wp-content/uploads/2020/08/plasmid10.png)
E.coli Plasmid #10 (N-terminal signal peptide for membrane protein expression, C-terminal CL7, N-terminal HIS ) • NUCLEUS BIOTECH
![SOLVED: For the following experiment, we want the His-Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers so that the SOLVED: For the following experiment, we want the His-Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers so that the](https://cdn.numerade.com/ask_images/4221f2a75b994af38ac8431ee92ae69a.jpg)
SOLVED: For the following experiment, we want the His-Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers so that the
Figure 1 | Effects of N-Terminal and C-Terminal Polyhistidine Tag on the Stability and Function of the Thermophilic P450 CYP119
![General representation of our modified pACE vectors with an N-terminal... | Download Scientific Diagram General representation of our modified pACE vectors with an N-terminal... | Download Scientific Diagram](https://www.researchgate.net/publication/260950545/figure/fig2/AS:601723106250752@1520473376097/General-representation-of-our-modified-pACE-vectors-with-an-N-terminal-tag-left-panel.png)